Doctor homeopath of the Ryazan Medical Center “Similia”, Ph.D., assistant of the Department of Matophysiology of the RyazGMU named after Academician I.P. Pavlova
Homeopathy is a science that studies the human body as a whole and correctly applies the law of similarity to stimulate the vital energy of man .
In the very word “homeopathy”, consisting of two Greek words “homois” similar and “pathos” disease, the basic law of homeopathy is hidden: similar is treated similar [1,10,11].
The German doctor Samuel Hahnemann formulated the main principles of the homeopathic method of treatment. He in 1796 in his medical work “Experiment of a new principle of finding healing properties of medicinal substances” published his conclusions on the first fundamental principle – the law of similarity. Hahnemann’s genius is that he was the first in the world to formulate the law of the similarity – like treated like [1, 10, and 11].
In the work, “Organon of Medical Art” Hahnemann described in detail four basic provisions of the homeopathic method of treatment. Hahnemann came to the law of similarity, because he was a brilliant experimental scientist, thanks to the experiment with a cinnamon crust, he proved that under normal conditions, china is capable of causing fever attacks that are characteristic of malaria, and in the pathological conditions of chin, respectively, cures according to the law of similarity. The bark of a cinchona is described in Cullen’s “Materia Medica”. In the chapter on the healing effect of the cinchona bark, Cullen expounded the long-known thesis that the effectiveness of this drug in malaria is explained by its “tonic effect on the stomach, as an astringent.” Hahnemann completely disagreed with the author, so Hahnemann decided to test the effect of the cinchona bark on himself, eventually opening with the help of the “allopathic similar preparation” the law of similarity [10, 11].
It must be said that Hahnemann was not the first to experience the effect of drugs on a healthy body and came to the idea of the law of similarity.
We all know Hippocrates well and boldly call him the father of medicine. He lived much earlier than Hahnemann did and, as a doctor-philosopher was a homeopath. It belongs to him the words “similar things should be treated with similar things, vomiting should be treated with emetics” .
A famous physician Paracelsus, who lived in the late 16th – early 16th centuries, again developed a homeopathic idea, almost forgotten from the time of Hippocrates. “Never,” he said, “the opposite heals the opposite, but alike always treated alike” .
Kravkov Nikolai Pavlovich – the founder of Soviet pharmacology, was born in Ryazan. In 1924, he conducted an experiment and proved that high dilutions of metal salts and poisons in 10-32 exert a noticeable effect on the vessels of the rabbit ear and on the pigment cells of the living frog, and wrote a paper “On the limits of sensitivity of living protoplasm” .
Until now in scientific circles and among doctors, there is a mistrust towards homeopathy, misunderstanding of the law of similarity, homeopaths are sometimes considered charlatans, instead of doctors, and medicines are called placebos. The positive effect of homeopathic preparations is explained by the placebo effect . To prove the law of similarity to the modern world, we need a model of the disease [8, 9] to exclude the placebo effect – it is necessary to carry out the experiment on animals, this requires a similar preparation in high dilution and in a potentiated form. The resulting material must be subjected to histological, morphometric and statistical studies. If all these conditions are met, the similarity law will be proved at the morphological level, which is very important for the further development of homeopathy in the world .
All these conditions we fulfilled in the conditions of the experiment and proved the similarity law at the cellular level . The purpose of this work is to study the influence of homeopathic nanodilution on the state of the body and mammary gland of white, non-native virgin female rats under normal conditions and in cystic mastopathy in an experiment.
As a model of the disease, we chose cystic mastopathy, which was caused by synestrol (synthetic analogue of estrogens) for 45 days, as a similar preparation we chose homeopathic dilutions of veroshpiron, which caused complete regression of the cysts. Professor P.A. Chumachenko developed the model of cystic mastopathy at the Department of Pathological Anatomy of the RyazGMU [3, 6, 7, 8, and 9].
In 2000, in the experiment we randomly found the therapeutic effect of veroshpiron in cystic mastopathy. I must admit that veroshpiron (potassium saving diuretic) was chosen for the experiment with a different purpose – we wanted to get a model of breast cancer in laboratory animals. After reading the description of the action of veroshpiron in the annotation and in the literature, we found out that veroshpiron can cause mastopathy, gynecomastia and even breast cancer as side effects in women, so we started using veroshpiron after creating cystic mastopathy in rats [3, 5, 6, and 7]. In the candidate’s thesis, the following data were obtained: when studying material doses of veroshpiron in an intact series that means animals received veroshpiron under normal conditions, veroshpiron expanded the passages, increased glandular secretion, and in cystic mastopathy caused narrowing of the passages to normal, healing the cystic mastopathy. What is the mechanism of action of veroshpiron – if it normally extends the passages, and with cystic mastopathy, it narrows them back to normal? This is the essence of the law of similarity – the like is treated like that. Veroshpiron was similar to the synestrel model of cystic mastopathy . To prove the consistency of homeopathic preparations and the effect of homeopathic doses at the cell level, we decided to repeat the experiment with veroshpiron, to make homeopathic nanodilutions from it and to conduct a repeated experiment. In 2007, we offered our idea to the rector of the International Academy of Classical Homeopathy, Professor George Vithoulkas, so we decided to work together, as a result of our studies in 2010, our joint monograph “Scientific proof of the similarity law in homeopathy (experimental research)” was published /
With the help of this work, we managed to bring homeopathy to the level of evidence-based medicine .
MATERIAL AND METHODS
We studied the mammary glands of 200 white mongrel virgin females of rats that were conditionally divided into 7 experimental groups and 9 control groups. The work with animals was carried out in accordance with the European Convention for the Protection of Vertebrates used for experimental and other scientific purposes”(Strasbourg, 1986), Order of the Ministry of Health and Social Development of the Russian Federation of August 23, 2010 and 708n” On Approval of Regulations laboratory practice “and the order of the Ministry of Health of the USSR of 12.08.1977, № 755 “On measures to further improve the organizational forms of work using experimental animals.” Experimental groups after receiving the model of cystic mastopathy received homeopathic veroshpiron orally for a month according to the schemes, control groups – group 1 control of cystic mastopathy and group 2 control of cystic mastopathy – a month after developing it. Separately there was a group of intact animals that did not receive drugs (control of the norm), the group after receiving the model of cystic mastopathy received placebo-empty granules. After the expiration of the experiment, rats were slaughtered under ether anesthesia; the mammary glands were removed, fixed in a 10% formalin solution. Paraffin, all sections stained with hematoxylin-eosin, selectively hematoxylin and picrofuxin, sudan3 for lipids, for elastic according to Weigert, impregnated silver with reticular fibers according to Futu. Further, the study of histological preparations was carried out using the following methods:
- General morphological description.
- Morphometric analysis.
- Statistical. The morphometric method included 23 indicators (Russian).
Homeopathic dilutions of C6, C12, C30, and C200 were made from the allopathic veroshpiron in the Ollo pharmacy (Moscow). Professor D. Vithoulkas suggested schemes for the introduction of homeopathic veropyron.
Seven experimental groups:
First group – 6s 3 times a day for 1 month;
Second group – 6c, 12c, 30c per day 1 month. – “wave-like scheme of introduction”;
Third group – 30s 3 times a day for 1 month;
Fourth group – 30c once a week;
Fifth group – 6c 3 times a day – 10 days, 12c 3 times a day – 10 days, 30c 3 times a day – 10 days;
Sixth group – 200s 3 times a day 1 month;
Seventh group – intact animals 6c-14 days, 12c – 14 days 3 times a day 1 month.
RESULTS OF OWN RESEARCH
Normally, the mammary glands consist of collapsed passages with cubic epithelium, which are located in the adipose tissue, poor in blood vessels and cells.
Fig. 1. Normal rat mammary glands (staining with hematoxylin and eosin, volume 8, app.7) When rats were given material doses of veroshpiron for 2.4, 8 weeks in some cases, especially when receiving maximum doses of veroshpiron for two weeks and minimum doses for 8 weeks.
Fig. 2. Mammary glands of rats of the intact series when receiving material doses of veroshpiron for 8 weeks (staining with hematoxylin and eosin)
We observed an interesting picture: the passages were arranged in groups of 5-10 passages, the epithelium was cubic, the lumens of the passages were clearly distinguishable, with some passages slightly enlarged, having irregularly shaped lumens, and a little secret in the lumens. This phenomenon can be regarded as a phenomenon of proving veroshpiron and some mammothropic effect on the body, that is, the material doses cause some kind of disease of the mammary glands. Based on the morphometry, we found that veroshpiron during 2, 4, and 8 weeks of use increases the rate of PS, with some indicators increasing significantly compared with the control (p <0.05), the maximum dose of veroshpiron increases significantly the rate of EC for 4 weeks, veroshpiron in material doses affects the size of the nuclei of the EC. All the parameters of the PS, EH, EC of the intact series are shown in Fig. 3 in the form of diagrams, which also speaks of the stimulating mammotropic action of veroshpiron in the mammary glands of rats.
Fig. 3. Intergroup morphometric parameters: average passage size [DS] epithelial height (EH), epithelial cariometry (EC) of an intact series of rats upon obtaining material doses of veroshpiron
Fig. 4. Mammary glands with sinestrol injection for 6 weeks in the control groups – a model of cystic mastopathy (staining with hematoxylin and eosin)
When receiving the model of cystic mastopathy (Figure 4), the passages were located in small groups; the passages were sharply expanded to the formation of small, medium and large cysts, epithelium flattened, in the lumens of cysts, protein-fat masses and cells of condensed epithelium. After 2, 4, 8 weeks from the moment of obtaining the model of cystic mastopathy in the control groups, the morphological signs of cystic mastopathy do not disappear – we also observed cysts of different sizes with a flattened epithelium.
Fig. 5. Mammary glands of control rats after 4 weeks from the moment of obtaining the model of cystic mastopathy (staining with hematoxylin and eosin)
Objectively, when sinestrol was administered, rats became sluggish, drowsy, ate poorly, some lost weight, a watery stool appeared on the skin there was a tick-like lesion, especially in the ears and nose. Palpation in the abdominal wall palpated breast cysts. According to the morphometry data, virtually all indicators, in particular, PS, EH, EC, were significantly changed.
Intergroup morphometric parameters: average size of passages (DS), epithelial height (EH), epithelial cariometry (EC) in cystic mastopathy with the introduction of homeopathic veroshpiron of experimental groups
When obtaining homeopathic dilutions of veroshpiron in the intact conditions for 4 weeks (6C – 2 weeks, 12C – 2 weeks), mammary glands of rats had a normal structure.
Fig. 7. Mammary glands with the introduction of homeopathic veroshpiron in the intact conditions for 4 weeks (staining with hematoxylin and eosin)
Veroshpiron in homoeopathic doses had no effect on the mammary glands of rats; we did not receive proving in homeopathic doses of veroshpiron in normal conditions, which is very important.
In experimental groups with the introduction of homeopathic dilutions of veroshpiron for a month, the rats were objectively different from the control animals (control of cystic mastopathy), which were not given homeopathic veroshpiron: the animals under the action of the drug “came to life in front of their eyes,” became more active, ate well, at end of the month on the tick-borne lesions started to disappear, which indicates an increase in immunity. In control this was not observed, the animals were sluggish, some even died. Histologically, the mammary glands had the following structure in the preparation of 6C in group 1 of the experiment with CM: the passages were somewhat enlarged with the cubic epithelium, the lumens were clearly discernible, and in general a picture of incomplete regression of the cystic process.
Fig. 8. Mammary glands with the introduction of potency 6C veroshpiron in cystic mastopathy for 4 weeks (staining with hematoxylin and eosin, volume 8, about 7)
According to the morphometry data, the PS index significantly decreases compared to the control of the CM model (p <0.05), compared to the control of CM in 4 weeks from the moment of its receipt it has little changes, and compared with the norm control – it is irrelatively increased, the process of regression of cysts is active, but it is slow and incomplete.
With the “wave-like” scheme of administering veroshpiron in the second group of the experiment (6C – morning, 12C – lunch, 30C evening). This scheme for the introduction of a homeopathic preparation is not recognized by all homeopaths, for comparison a very interesting experimental group. The passages were located in large groups of 20-30 passages in one group, forming lobes, passages narrowed, lumens distinguishable, epithelium cubic.
Fig. 9. Mammary glands with a wave-like scheme of administration of homeopathic veroshpiron for 4 weeks (staining with hematoxylin and eosin)
According to the morphometry data, the PS index was significantly lowered compared with the control of CM (p <0.05), but compared with the norm control, the index was significantly increased (p <0.05), normalization of CE did not occur, and CE was significantly increased. Regression of cysts is better than in the first group, but not all indicators are normal. When 30C was administered three times a day in experimental group 3 histologically, we observed a picture of pronounced regression of the cysts; passages narrowed, epithelium cubic, stroma full blood vessels.
Fig. 10. Rat mammary glands with 30C potency administered daily for 4 weeks with cystic mastopathy (staining with hematoxylin and eosin)
With a weekly potency of 30C with CM in the four group of the experiment, the passages were arranged in groups, some passages dilated, other passages narrowed, but the epithelium was cubic, it means that histologically, we observed incomplete regression of the cysts.
Fig. 11. Rat mammary glands with weekly potency of 30C veroshpiron in cystic mastopathy, incomplete regression of cysts (staining with hematoxylin and eosin).
In terms of morphometric parameters in group 3, PS significantly decreases in comparison with CM control (p <0.05), but it is elevated (p <0.05) compared to norm control, significantly increased EH compared with cyst control (p < 0.01), compared with the norm control it was reduced (p <0.01), significantly increased EC compared with the control of the cyst-model and control of the norm (p <0.01).
According to the morphometry in the 4th group of the experiment, PS was significantly decreased (p <0.05), but compared to the control of CM in 4 weeks – there are no changes, compared to the norm control – the indicator is elevated, but unreliable; EH is significantly increased in comparison with the control model (p <0.05), compared with the control of cysts after 4 weeks – the indicator reacts poorly, compared with the norm control – significantly lowered (p <0.01); significantly increased CE in comparison with the control of the cyst-model and the control of the norm (p <0.00 1), (p <0.05).
With the introduction of homeopathic veroshpiron in the 5th group – the scheme of a gradual increase in doses during the month (6C – 10 days, 12C – 10 days, 30C – 10 days) – clinically the animals also gradually and gently recovered and recovered with increasing dilution and potencies . Histologically, the mammary glands in all studied cases of the experiment had a pronounced regression of the cysts-the passages narrowed, the epithelium cubic, in the lumens the residual secret.
Fig. 12. Mammary glands of rats with the introduction of homeopathic veroshpiron in cystic mastopathy – gradual increase in potencies of 6C, 12C, 30C for a month (staining with hematoxylin and eosin).
According to the morphometry data: the PS was significantly lowered compared to the control of the CM model (p <0.01) and CM control 4 weeks after the model was obtained (p <0.05), and compared with the norm control, the indicator was slightly elevated, but the data are unreliable, which indicates a pronounced regression of cysts in this group of experiences; significantly increased EH compared with the control groups of CM (p <0.001), compared with the control of the rate slightly increased; significantly increased CE compared with the control of cysts – model CM (p <0.001) and control norm (p <0.01).
With the introduction of homeopathic veroshpiron C200 in the 6th group of the experiment, histologically the mammary glands had the following structure. In all cases of the experiment, complete regression of cystic mastopathy was observed – the passages were sharply narrowed, the lumens were not distinguishable, the epithelium was cubic.
Fig. 13. Mammary glands of rats with the introduction of 200C veroshpiron in cystic mastopathy, pronounced regression of cysts (staining with hematoxylin and eosin)
According to the morphometry data: PS significantly decreases in comparison with the control of the cysts – the CM model (p <0.01) and cyst control after 4 weeks (p <0.05), in comparison with the norm control – complete normalization of this indicator, which indicates complete regression of cysts, that is, cystic mastopathy; significantly increases EH compared with the control of cysts (p <0.001), significantly increased EC compared with the control of cysts – the model and control of the norm (p <0.01).
Fig. 14. Mammary glands of rats with placebo administration in cystic mastopathy (staining with hematoxylin and eosin)
Intergroup changes in the indicators of PS, EC, and EC are shown in Figure 6 in the form of diagrams. Separated from the experimental groups, we conducted a group of experiments after obtaining the model of CM. Rats received placebo empty granules for a month (placebo-controlled animals). Histologically and morphometrically, the picture typically matches with the second group of CM control – that is, we observed cystic mastopathy, which does not change in any way compared to the model control (Figures 14, 15). This group has proved the scientific fact that homeopathy is not a placebo effect.
Fig. 15. Change in the morphometric index of PS (passage size) in all study groups
- Veroshpiron in homeopathic nanodilution exerts its influence on the mammary glands in cystic mastopathy.
- In many cases, the degree of regression of the cysts has reached a high level in comparison with the control.
- In single observations, especially with the action of low potencies of veroshpiron, regression is incomplete, but under the action of veroshpiron, the epithelium is restored to normal.
- According to the morphometry data, homeopathic veroshpiron in most cases causes a pronounced regression of the cysts, the higher the dilution, the more intensive the treatment effect, the better the potency C200, the complete regression of the cysts in all cases. 5. Veroshpiron in homeopathic doses does not affect the intact mammary gland, but in material doses, it has a stimulating mammothropic effect, which resembles the picture of proving a kind of “synestral model” of cystic mastopathy.
- According to the mechanism of action, veroshpiron is an organotropic drug with synestrel model of cystic mastopathy; veroshpiron can be further studied and used in the treatment of women from cystic mastopathy, especially if there is an application of hormones during pregnancy and childbirth, with the purpose of contraception.
THE IMPORTANCE OF WORK
In this scientific study, the project and plan of which was developed in conjunction with the rector of the International Academy of Classical Homeopathy George Vithoulkas, in an animal experiment using morphometry, we managed to bring homeopathy to the level of evidence-based medicine, and we proved the law of similarity at the cellular level with the help of homeopathic veroshpiron – similar organotropic potentiated preparation. At the time of Hahnemann, there was no modern morphology, no pathophysiology, and, especially, microscopy and morphometry, so 200 years after Hahnemann’s discovery of homeopathy, we managed to conduct these unique experiments on animals. To all of humanity, we can confidently state now that homeopathy is not a placebo effect, and homeopathic preparations are not placebo. Homeopathy is a great science, based on the law of similarity and the effect of nanodilution of the drug on the cell and on the body as a whole. Treatment with the help of homeopathy is an art; curing any pathological process occurs according to the LAW OF SIMILARITY! The future of medicine for homeopathy!
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